Maximal ethanol accumulation capacity and ethanol tolerance of cell proliferation in the superior parent and its segregant.

<p>(A) Identification of a segregant with the same high ethanol accumulation capacity of CBS1585. A segregant, Seg5 (n), derived from CBS1585 (2n) showed better attenuation of the fermentation medium compared to the laboratory strain BY710. The diploid (Seg5/BY710) showed similar final attenuation as the superior strains CBS1585 and Seg5. Strains: (•) Seg5, (○) CBS1585, (▪) Seg5/BY710 and (□) BY710. (B) Maximal ethanol production capacity in 250 mL of YP+33% glucose at 25°C. The strains CBS1585 (2n), Seg5 (n), Seg5/BY710 (2n) showed much higher ethanol accumulation capacity compared to BY710 (n). (C) Growth assays on plates containing YP or YPD plus ethanol (18 and 20% v/v). The strains CBS1585 (2n), Seg5 (n), Seg5/BY710 (2n) showed much higher ethanol tolerance of cell proliferation compared to BY710 (n).