Loss of function assessment and complementation assay with the causative gene <i>URA3</i> in QTL3.

<p>(A) <i>URA3</i> loss-of-function assay. The strain Seg5/BY710 (•) had its <i>URA3</i> copy deleted, Seg5-ura3Δ/BY710 (○). Both strains were tested in 250 mL of YP+33% glucose at 25°C. (B) <i>URA3</i> complementation study. The <i>URA3</i> auxotrophic strain BY4741-ura3Δ (▪) had the <i>URA3</i> gene inserted in its original position, BY4741-<i>URA3</i> (□). The performance of both strains were assessed by semi-static fermentations in 250 mL of YP+33% glucose at 25°C. (C) Determination of ethanol tolerance of cell proliferation with the hybrid diploid strains Seg5/BY710-ura3Δ, Seg5-ura3Δ/BY710-ura3Δ.