FcRγ-expressing DN T cells are a distinct effector-memory subset in both B6 and LPR mice.

<p><b>A.</b> Freshly isolated B6 (n = 3, left column) and LPR (n = 4, right column) splenocytes were stained for TCRβ, CD4, CD8, NK1.1, CD16/32, CD44, and CD62L expression and examined by flow cytometry. Within the DN T cell gate (TCRβ⁺, CD4⁻, CD8⁻, NK1.1⁻), expression of CD44 (top row) and CD62L (bottom row) in the CD16⁺ (shaded) and CD16⁻ (unshaded) subsets was plotted. <b>B.</b> Median fluorescence intensity (MFI) of CD44 staining in CD16⁺ and CD16⁻ DN T cells for all 7 mice is shown. Unpaired t tests p = 0.006 (CD16⁺ vs. CD16⁻ B6 DN T cells) and p = 0.0009 (CD16⁺ vs. CD16⁻ LPR DN T cells). <b>C.</b> MFI of CD62L staining in CD16⁺ and CD16⁻ DN T cells for all 7 mice is shown. Unpaired t tests p = 0.0036 (CD16⁺ vs. CD16⁻ B6 DN T cells) and p = 0.0165 (CD16⁺ vs. CD16⁻ LPR DN T cells).