CLEC4F⁺ cells were appeared in the liver environment under Kupffer cell depletion and inflammatory stage.

<p>(A) Kupffer cells were depleted by Cl₂MBP-encapsulated liposome by intravenous injection (100 µl/mouse) at day 0 and livers were harvest at day 1, 7, 14 and 28. F4/80 and CLEC4F immunohistochemistry of liver sections were performed. (B) The numbers of F4/80⁺ or CLEC4F⁺ cells in livers were shown. For generating inflammatory stage, wild-type and <i>Clec4f−/−</i> littermates were infected with <i>L. monocytogenes</i> (1×10⁵ CFU/mouse) intravenously. (C) The numbers of F4/80⁺ or CLEC4F⁺ cells in livers during <i>L. monocytogenes</i> infection. (D) Immunohistochemistry of <i>L. monocytogenes</i> infected livers of wild-type and <i>Clec4f−/−</i> mice at day 5 after infection. (E) Kaplan-Meier survival curves were shown for <i>Clec4f−/−</i> or wild-type littermates with <i>L. monocytogenes</i> infection. The <i>p</i> value was determined by Log-rank test.