<p>RNA in situ hybridisation reveals CG42355-LacZ reporter construct expression in control testes, and no signal in <i>Nxt1<sup>Z2-0488</sup></i>/<i>Nxt1<sup>DG05102</sup></i> or <i>comr</i> mutant testes. Addition of one intron generates detectable, although weak, expression in <i>Nxt1<sup>Z2-0488</sup></i>/<i>Nxt1<sup>DG05102</sup></i> testes, but not in <i>comr</i> mutant testes.</p
<p>(A) Top: Intron-exon structure of the <i>nup98-96</i> locus. Coding region in dark grey. Gene pro...
<p>(A) Schematic representation of <i>Dicer1</i> and <i>Dgcr8</i> loci. Exon 24 of the <i>Dicer1</i>...
<p>(A) Heat map showing the clustering of de-regulated transcripts identified through RNA-Seq analys...
<p>(A) Schematic diagram to illustrate reporter construct design. Promoter regions are narrow colour...
<p>Comparison of gene expression defects in Nxt1, <i>aly</i> (<i>aly</i>-class mutant) and <i>nht</i...
<p>(A) Expression of nascent (unspliced) RNA was quantified using primer pairs a+c, b+c or b+d, whil...
<p>(A) Expression of an <i>Nxt1</i> RNAi hairpin construct in the male germline causes a highly pene...
<p>Semiquantitative RT-PCR was carried out using total RNA isolated from D7, D14, D21, and D35 teste...
<p>(A) Expression of individual retrotransposons in <i>Pld6</i> KO and WT testes. Those showing incr...
<p>RNAs were prepared from adult testes and hybridized with radiolabeled probes specific for pachyte...
<div><p>The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export o...
<p>Semiquantitative RT-PCR for the 51 intronless genes identified in <a href="http://www.plosone.org...
The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most m...
<p>Semiquantitative RT-PCR analyses for the RNA isolated from Sertoli cell cultures of AMH-<i>Cre</i...
<p>(A) All stages of spermatogenesis are seen in wild type testes by phase contrast microscopy. Larg...
<p>(A) Top: Intron-exon structure of the <i>nup98-96</i> locus. Coding region in dark grey. Gene pro...
<p>(A) Schematic representation of <i>Dicer1</i> and <i>Dgcr8</i> loci. Exon 24 of the <i>Dicer1</i>...
<p>(A) Heat map showing the clustering of de-regulated transcripts identified through RNA-Seq analys...
<p>(A) Schematic diagram to illustrate reporter construct design. Promoter regions are narrow colour...
<p>Comparison of gene expression defects in Nxt1, <i>aly</i> (<i>aly</i>-class mutant) and <i>nht</i...
<p>(A) Expression of nascent (unspliced) RNA was quantified using primer pairs a+c, b+c or b+d, whil...
<p>(A) Expression of an <i>Nxt1</i> RNAi hairpin construct in the male germline causes a highly pene...
<p>Semiquantitative RT-PCR was carried out using total RNA isolated from D7, D14, D21, and D35 teste...
<p>(A) Expression of individual retrotransposons in <i>Pld6</i> KO and WT testes. Those showing incr...
<p>RNAs were prepared from adult testes and hybridized with radiolabeled probes specific for pachyte...
<div><p>The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export o...
<p>Semiquantitative RT-PCR for the 51 intronless genes identified in <a href="http://www.plosone.org...
The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most m...
<p>Semiquantitative RT-PCR analyses for the RNA isolated from Sertoli cell cultures of AMH-<i>Cre</i...
<p>(A) All stages of spermatogenesis are seen in wild type testes by phase contrast microscopy. Larg...
<p>(A) Top: Intron-exon structure of the <i>nup98-96</i> locus. Coding region in dark grey. Gene pro...
<p>(A) Schematic representation of <i>Dicer1</i> and <i>Dgcr8</i> loci. Exon 24 of the <i>Dicer1</i>...
<p>(A) Heat map showing the clustering of de-regulated transcripts identified through RNA-Seq analys...