Par6C and Rnd3 are essential mediators of BDNF-induced spinogenesis in organotypic slice cultures.

<p>Organotypic hippocampal slice cultures were transfected on DIV2 with Tomato (TFP) (Control and BDNF), A) ± Par6C, ± Par6C+caRhoA, ± Par6C+sh-p190GAP ± sh-Par6C. B) ± Rnd3, ± Rnd3+caRhoA, Rnd3+sh-p190GAP, ± si-Rnd3, and then stimulated with ±50 ng/mL BDNF on DIV4 until fixed on DIV6. Dendrites of CA1 pyramidal neurons were imaged, and a single dendrite was analyzed per neuron (30–60 neurons/condition in 3 separate experiments). (± SEM, Statistical analyses utilized ANOVA and Tukey’s post-test (*p<0.001 compared to control, #p<0.001 compared to BDNF, ¥p<0.001 compared to Par6 or Rnd3). E) Model of BDNF- and CREB-dependent synaptogenesis.