Enrichment of DNA repair markers in the pseudo XY bodies of <i>Spo11^+/+</i> oocytes.

<p>(A) Oocyte nuclei from <i>Spo11^+/+</i> E17.5 embryos were immunostained with anti-SYCP3 (red), anti-γH2AX (blue) and anti-RAD51 (green, upper panel) or anti-DMC1 (green, middle panel) or RPA (green, lower panel). Foci of each marker listed above are indicated with arrowheads. Numbers in the bottom left corner of every picture represent the percentage of nuclei of the respective type in the analyzed cell population. Quantification of the four fractions (defined in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003538#pgen-1003538-g003" target="_blank">Figure 3A</a>) was performed in 271, 54, and 53 oocyte nuclei, for RAD51, DMC1, and RPA protein foci, respectively. (B) The number of RAD51, DMC1, and RPA foci was counted in pachytene oocyte nuclei showing both a pseudo XY body and foci (red circle). The average total number of foci of each protein per nucleus is reported in the first column of the table. The second and the third column show the percentage of foci located within a pseudo XY body-like domain and the percentage of pseudo XY body-like domains which contained at least one focus.