SATB1 binding to Sox2 is enhanced by Akt signaling.

<p>(<b>A</b>) SATB1 associates with Sox2, but not Oct4 or Klf4. (<b>B</b>) Endogenous interaction between SATB1 and Sox2. SATB1 was immuprecipitated from F9 cell lysates, immuprecipitations were subjected to immunblotting with anti-SATB1 and anti-Sox2. (<b>C</b>) Mapping of SATB1 domain required for Sox2 binding using GST pull-down assay. (<b>D</b>) A schematic representation of Flag-GFP-tagged Sox2 constructs is shown. (<b>E</b>) Mapping of Sox2 domains that are necessary for SATB1 binding. (<b>F</b>) SATB1S47A sequesters much less Sox2 than wild-type SATB1 and SATB1S47D. (<b>G</b>) Inhibition of Akt signaling disrupts SATB1/Sox2 interaction. HEK293T cells were co-transfected with Myc-SATB1 and Flag-Sox2, serum-starved and treated as indicated.