Establish non-toxic MTX and MMF dose regimens for <i>in vivo</i> selection.

<p>6–8 week old NSG mice (n = 6) were (<b>a</b>), administered MTX by i.p. injection at 25 mg/kg/day twice a week the first week (days 1 and 4) and only once the second week (day 8), and/or (<b>b</b>), fed 0.563% MMF medicated feed for 2 weeks. Serum MTX and MPA levels in each case were analyzed by HPLC. Drug toxicity was then monitored by measuring (<b>c</b>), white blood cell counts, (<b>d</b>), body weight, (<b>e</b>), Hemoglobin, (<b>f</b>), ALT, and (<b>g</b>), creatinine levels. (c–g), Mean levels of each measurement (± S.D) after the last treatment (i.e., at day 14) are depicted. There was no significant difference between control and treatment mice (p≥0.05). Data were evaluated between control and treatment mice using an unpaired, two-tailed Student’s t - test.