mRNA expression of <i>Mas</i>, <i>AT1</i> and ACE2 in the kidneys after injection of adriamycin.

<p>In A, <i>Mas</i> and <i>AT1</i> mRNA levels were evaluated in the kidney before (Sham) and 7, 14 and 21 days after injection of adriamycin (ADR, 10 mg/kg). In B, <i>Mas</i> and <i>AT1</i> mRNA levels at day 14 in kidneys of mice injected with adriamycin that were treated with vehicle (VE) or losartan (LOS, 10 mg/kg). In C, ACE2 mRNA levels at day 14 in kidneys of mice injected with adriamycin that were treated with vehicle (VE) or losartan (LOS, 10 mg/kg). The dotted line across the graphs represents levels in control animals. Renal mRNA levels of receptors and ACE2 were estimated by real time PCR. Results are mean ± SEM of n = 5 mice per group. (*) for P<0.05 when compared to sham group.