Western blot analysis of the expression of Bcl-2, c-myc, AKT, p-Akt, p-NF-κB in Jurkat EL4 and Hut78 cells treated with 60 µg/ml solvent control or 13-MTD for 2, 6, 12, 24 h.

<p><b>GAPDH was used as a loading control.</b> There was no change in the expression of Bcl-2, c-myc or AKT proteins. The expression of phosphorylated AKT and NF-κB were decreased in a time-dependent manner with 13-MTD treatment. All data are derived from three individual experiments with triplicate wells. (B) These three cell lines were treated with solvent or 13-MTD (60 mg/ml) for 24 hours, followed by Akt inhibitor V (40 uM) exposure for 30 min. The phosphorylation of AKT was significantly inhibited by Akt inhibitor V. The anti-growth (C) and apoptosis-promoting (D) effects of 13-MTD on T-NHL cells almost disappeared once the phosphorylation of AKT was inhibited. Result are representative of there independent experiments. All the values represent means ±standard deviation (S.D.).