TGFβ represses miR-127 expression by enhancing c-Jun activity.

<p>(<i>A</i>) qPCR analysis of MMP13 (left), c-Jun (middle), and miR-127 (right) expression in MHCC97H cells treated with TGFβ. *p<0.01, TGFβ (+) <i>vs.</i> TGFβ (-) group. (<i>B</i>) Transient transfection assay. Hela cells were co-transfected with miR-127 promoter (pro) luciferase (luc) reporter, and c-Jun or c-Fos expression plasmids. *p<0.01, c-Jun (+) <i>vs.</i> c-Jun (-) group. (<i>C)</i> Transient transfection assay. Hela cells were co-transfected with miR-127 or AP1 promoter reporter along with c-Jun/c-Fos plasmid (100 ng) in the absence or presence of TGFβ (5 ng/ml). *p<0.01, c-Jun/Fos <i>vs.</i> control without (-) or with (+) TGFβ; ^¥p<0.01, control with (+) TGFβ <i>vs.</i> control without (-) TGFβ. (<i>D</i>) Transient transfection assay. Hela cells were co-transfected with miR-127 promoter reporter, c-Jun (100 ng) and/or p53 plasmids (100 ng). *p<0.01, c-Jun or p53 <i>vs.</i> control; ^¥p<0.01, c-Jun/p53 <i>vs.</i> p53. (<i>B-D</i>) Luciferase (Luc) activity was normalized by Renilla activity (act). (<i>E</i>) qPCR analysis of miR-127 and p53 expression in HepG2 cells transfected with control (con) or p53 siRNAs (si-p53) (20 nM), or in HCT116<i>p53^+/+</i> (+) and HCT116<i>p53^−/−</i> (-) cells. *p<0.01, si-p53 <i>vs.</i> control. (<i>F</i>) qPCR analysis of miR-127 expression in MHCC97H cells that were overexpressed with c-Jun and/or p53. *p<0.01, p53 <i>vs.</i> control (-); ^¥p<0.01, c-Jun/p53 <i>vs.</i> p53.