Effect of hydrogen peroxide on compartmental <i>E</i>_GSH and pH.

<p>A-C) Cells were pregrown in SC_URA (48 h; 30°C; 600 rpm), inoculated in SC_URA (A₆₀₀ = 0.001) and grown (25°C; 600 rpm) until exponential phase (A₆₀₀ = ∼0.5). Cells were then treated with various doses of hydrogen peroxide for 30 min and pH and redox environment analyzed. (A cytosolic pH and <i>E</i>_GSH, B) mitochondrial matrix pH and <i>E</i>_GSH, C) peroxisomal pH and <i>E</i>_GSH. 10,000 cells were counted for each sample. Each experiment was conducted in triplicate with error bars representing the standard deviation of three experiments. Error bars may be difficult to visualise as the errors are in the ∼5 mV range.