Dynamic recovery of cells after hydrogen peroxide treatment.

<p>Cells were pregrown in SC_URA (48 h; 30°C; 600 rpm), inoculated in SC_URA (A₆₀₀ = 0.001) and grown (25°C; 600 rpm) until exponential phase (A₆₀₀ = ∼0.5). Starting <i>E</i>_GSH was measured then cells were treated with hydrogen peroxide (2 mM; 20 min; 25°C), washed and resuspended in fresh medium, and pH and redox state analyzed by flow cytometry at two-minute intervals. A) cytosolic pH and <i>E</i>_GSH, B) mitochondrial matrix pH and <i>E</i>_GSH and C) peroxisomal pH and <i>E</i>_GSH. 10,000 cells were counted for each sample. Each experiment was conducted in triplicate with error bars representing the standard deviation of three experiments. The E_GSH and pH in untreated (control cells) is indicated by the marked dashed lines. Some error bars may be difficult to visualise as the errors are in the ∼5 mV range.