Direct epitope discovery of WNV epitope GTL9.

<p>(A) RP-HPLC profile of HLA-A*11:01 peptides from uninfected (blue) and WNV-infected (red) cells. (B) MS ion spectra of HPLC fraction 50 from uninfected (black) and infected (red) cells. MS data were recorded for 300 scans. (C) Overlay of the MS/MS fragmentation pattern of ion 466.7 in the infected HPLC fraction 50 (identified as the GTL9 peptide; red) with the MS/MS fragmentation pattern for the GTL9 synthetic peptide (black). (D) Florescence polarization competitive binding assay with the GTL9 synthetic peptide and HLA-A*11:01.