Experimental arrangements for imaging flow in spinner flask bioreactor.

<p>The flask was placed inside a rectangular housing filled with water to minimize refraction of laser light. The fluid in the spinner flask was seeded with 10<b> </b>µm glass particles and stirred by a stepper motor. Two beams illuminated the seeded flow and imaged by two high-speed CMOS cameras (IDT Y4), placed orthogonal to each other to map full 3D velocity profile of the flow.