hBMSC viability and proliferation on the surface of Ta and Ti coatings.

<p>(<b>A</b>) Quantification of Odyssey scanning of hBMSCs on the surface of Ta and Ti coatings stained with DRAQ5 in 48 h (t-test, assuming unequal variances, error bars represent ± SD, <i>n</i> = 3; Ti coating, 65.377±18.2972; Ta coating, 164.80±46.1212; Prob>|t|, 0.0498*). (<b>B</b>) Cell viability of hBMSCs on both coatings measured by PrestoBlue assay (t-test; error bars represent ± SD, <i>n</i> = 3; day 2; Ti coating, 0.45268±0.097954; Ta coating, 1.0767±0.495686; Prob>|t|, 0.992; day 3; Ti coating, 0.56950±0.089554; Ta coating, 1.26228±0.458399; Prob>|t|, 0.0620; day 4; Ti coating, 0.90275±0.082739; Ta coating, 2.13239±0.699593; Prob>|t|, 0.0390*; day 6; Ti coating, 0.86752±0.089092; Ta coating, 1.68688±0.386935; Prob>|t|, 0.0233*; the cell viability of hBMSCs on normal cell culture plate serviced as calibrator). (<b>C</b>) LIVE/DEAD Cell Viability Assays of hBMSCs on the surface of the coatings in 48 h. (<b>D</b>) Ki67 and DAPI stains of hBMSCs on the surface of the coatings in 48 h. (<b>E</b>) The flow cytometric analyses of hBMSC with CFSE on the surface of the coatings on day 7.