Suppression of gp96 expression decreases HBV production <i>in vitro</i>.

<p>Huh7 cells were co-transfected with pHBV and gp96-specific siRNA or luciferase-specific siRNA as mock. The secretion of HBsAg and HBeAg was determined by ELISA (A). The HBV DNA levels in the supernatant and cytoplasm were determined by real-time PCR (A) and Southern blotting (B). The HBV pgRNA and total RNA were analyzed by real-time PCR (C). The protein levels of gp96, hsp90, hsp70, GAPDH and actin were determined by western blotting (D). Data are presented as the means ±SD from three independent experiments. *P<0.05 and **P<0.01 compared to the mock.