<p>Cells were infected with all microorganisms at an MOI of 1. Phagocytosis was determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066898#s2" target="_blank">Materials and Methods</a> expressed as CFU/well or infection rate (%). Results from one representative donor measured in triplicate are shown and similar results were obtained with cells of at least three different donors. Statistical analysis was performed by pairwise comparison of GM-Mφ vs. M-Mφ using Student's <i>t</i>-test.</p
<p>(<b>A</b>) Suspensions of the bacterial strains indicated were analyzed for ability to bind human...
<p>Monocytes from CL patients (n = 9) and HS individuals (n = 6) were infected with <i>L</i>. <i>bra...
<p>Gating strategy used to assess phagocytosis of MOLT4 debris by MDM obtained by flow cytometry: co...
<p>Phagocytosis is measured as colony forming units (CFU) per well. Results from one representative ...
<p>Cells were infected with <i>Lm</i> at an MOI of 1. Results from one representative donor measured...
<p>Nuclei of macrophages are stained with DAPI (blue) and <i>Lm</i> was stained with a specific anti...
(A) CD14+ monocytes were cultured with 100 ng/ml IL-4, 80 ng/ml GM-CSF ± MDA-MB231-, HeLa-, HT29-, W...
<p>Histogram plots show macrophages incubated with latex beads (hatched area) and cells alone (black...
<p>CellTrackerTM Green-labelled parasites were added (10∶1) to THP-1-derived macrophages prelabelled...
Texto completo: acesso restrito. p. 1017–1024Leishmania spp. are intracellular protozoa residing in ...
<p>[<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005188#ppat.1005188...
<p>A) 1×10<sup>6</sup> monocytes/well were plated and then left untreated (control), GM-CSF-treated ...
<p>FITC-labelled bacteria were opsonised or not with human (IVIg) or bovine (bIgG) IgG. Subsequently...
<p><b>A. Determination of phagocytosis by color change method.</b> Phagocytosis of <i>M. genitalium<...
The evolution of cells of the monocyte-macropbage lineage (MML cells) in the spleen of Leishmania do...
<p>(<b>A</b>) Suspensions of the bacterial strains indicated were analyzed for ability to bind human...
<p>Monocytes from CL patients (n = 9) and HS individuals (n = 6) were infected with <i>L</i>. <i>bra...
<p>Gating strategy used to assess phagocytosis of MOLT4 debris by MDM obtained by flow cytometry: co...
<p>Phagocytosis is measured as colony forming units (CFU) per well. Results from one representative ...
<p>Cells were infected with <i>Lm</i> at an MOI of 1. Results from one representative donor measured...
<p>Nuclei of macrophages are stained with DAPI (blue) and <i>Lm</i> was stained with a specific anti...
(A) CD14+ monocytes were cultured with 100 ng/ml IL-4, 80 ng/ml GM-CSF ± MDA-MB231-, HeLa-, HT29-, W...
<p>Histogram plots show macrophages incubated with latex beads (hatched area) and cells alone (black...
<p>CellTrackerTM Green-labelled parasites were added (10∶1) to THP-1-derived macrophages prelabelled...
Texto completo: acesso restrito. p. 1017–1024Leishmania spp. are intracellular protozoa residing in ...
<p>[<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005188#ppat.1005188...
<p>A) 1×10<sup>6</sup> monocytes/well were plated and then left untreated (control), GM-CSF-treated ...
<p>FITC-labelled bacteria were opsonised or not with human (IVIg) or bovine (bIgG) IgG. Subsequently...
<p><b>A. Determination of phagocytosis by color change method.</b> Phagocytosis of <i>M. genitalium<...
The evolution of cells of the monocyte-macropbage lineage (MML cells) in the spleen of Leishmania do...
<p>(<b>A</b>) Suspensions of the bacterial strains indicated were analyzed for ability to bind human...
<p>Monocytes from CL patients (n = 9) and HS individuals (n = 6) were infected with <i>L</i>. <i>bra...
<p>Gating strategy used to assess phagocytosis of MOLT4 debris by MDM obtained by flow cytometry: co...