APE1 variants and predicted impact of amino acid change.

<p>Sorting intolerant from tolerant (SIFT) uses sequence homology to predict effects on protein function (<a href="http://sift.jcvi.org/" target="_blank">http://sift.jcvi.org/</a>). Scores <0.05 are considered deleterious, whereas those that are >0.5 are considered to be tolerated. The program polymorphic phenotypes (PolyPhen) predicts impact based on a set of empirical rules that apply to the protein’s sequence, phylogenetic and structural information (<a href="http://genetics.bwh.harvard.edu/pph/" target="_blank">http://genetics.bwh.harvard.edu/pph/</a>). Cologne University protein stability analysis tool (CupSat) predicts protein stability (<a href="http://cupsat.tu-bs.de/" target="_blank">http://cupsat.tu-bs.de/</a>), and was employed using the PDB APE1 protein structure (1DE8).</p>*<p>Described previously in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065922#pone.0065922-Hadi1" target="_blank">[33]</a>. N/A = not available. Once = it was observed a single time.