ATDC overexpression and knockdown in lung cancer cell lines.

<p>A. mRNA expression levels of ATDC analyzed by Real-time PCR in a panel of lung cancer cell lines. Note the highest expression is observed in A549 cell line and the lowest is seen in HBE cell. B. protein expression levels of ATDC analyzed by Western blot in a panel of lung cancer cell lines. Note a direct correlation of the protein level (B) with the transcripts (A) in each cell line. C. Real-time PCR analysis of ATDC overexpression efficiency in HBE cells and depletion efficiency in A549 and H1299 cells at a transcriptional level. D. Western blot analysis of ATDC overexpression efficiency in HBE cells and depletion efficiency in A549 and H1299 cells at a protein level. The band of lower MW in H1299 cells is a protein degradation product. Note a direct correlation of the protein level (D) with the transcripts (C) in each cell line.