TLR4 mediates A-SAA-induced cytokines and MMPs.

<p>Blockade of rhSAA (2.5 µg/mL)-induced cytokines and MMPs by specific inhibitors: lipoxin A4 (LX4; 1 µM), anti-SR-B1 antibody (5 µg/mL) and its isotype IgG1 control antibody (5 µg/mL), anti-RAGE (10 µg/mL) and its isotype IgG control antibody (10 µg/mL) and TAK242 (1 µM) for FPRL1, SR-B1, RAGE and TLR4 recepetors, respectively, on A) OA primary chondrocytes and B) OA FLS after 24h of stimulation. Cytokines and MMPs levels were quantified by ELISA in the culture supernatants. *a, statistically different from control. *b, statistically different from rhSAA-treated cells.