The expression of cytokines, chemokines and their receptors in the colons of DSS-treated mice.

<p>(A) The expression of cytokines, chemokines and their receptor mRNA in the colons of DSS-treated mice. Mice were sacrificed on day 8 and the colon tissues were harvested. Whole colonic RNA was isolated, reverse-transcribed into cDNA, and the expression levels of IL-1β, IL-6, IL-10, CCR2, CCR3, CCL2, CCL4 and CCL5 were determined by real-time quantitative PCR. Data are expressed as the ratios of the target mRNA levels to the HPRT mRNA level (n = 5 per group). *p<0.05. Data are presented as the means (SD). The data are representative of three independent experiments. (B) The intracellular staining profiles of IL-10 in the lamina propria CD4 T cells (CD4⁺), CD8 T cells (CD8⁺), macrophages (CD11b⁺, Gr-1^int) and dendritic cells (CD11c⁺) are shown as the percentages of cells in each area. The ratios of IL-10⁺ cells/total cells of each cell subset are shown over the area bar. The ratios of IL-10⁺ cells in each subset/total cells of all subsets are shown under the area bar. The results are representative of three independent experiments.