Characterization of vaccinia virus recombinants with EV gene deletions.

<p>DV-3 and recombinant vaccinia virus carrying deletions of the <i>A33R</i> gene (A33Rko), the <i>B5R</i> gene (B5Rko), or both <i>A33R</i> and <i>B5R</i> (A33Rko/B5Rko) were characterized for plaque formation and virus replication. (A) Plaque formation of viruses in BSC-40 cells after 7 days of incubation using both crystal violet staining and immunostaining methods. (B) Virus replication in BSC-40 cells. Monolayers were infected at a multiplicity of infection of 0.01 with DV-3 or each of the recombinant knockout viruses and total virus titer was determined by plaque assay at the indicated times after infection.