Antibody responses induced by different doses of VREP-FliC-D3.

<p>129sv/ew mice were immunized with β-Gal alone or with adjuvant, as indicated. Three different doses of VREP-FliC-D3 and VREP-OVA were used: 10⁶ IU, 10⁵ IU and 10⁴ IU. Control mice were given PBS. Each immunized group consisted of five mice, and four control mice were used. Serum was assayed for anti-β-Gal IgG, IgG1 and IgG2a by ELISA. A one-way ANOVA with Bonferroni post-hoc test was used to compare the response between mice given the same dose of adjuvant as well as between groups given adjuvant and the control group given β-Gal without adjuvant. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.