Contribution of type I IFN signaling.

<p><i>Ifnar1</i>^−/− (gray bars) and 129sv/ew (black bars) mice were immunized with β-Gal alone or with indicated adjuvant. 0.2 µg of soluble flagellin and 10⁶ IU of VREP constructs were used. Control mice were given VREP-OVA. Each immunized group consisted of five mice, and two control mice were used. Serum was assayed for anti-β-Gal IgG, IgG1 and IgG2a by ELISA. A one-way ANOVA with Bonferroni post-hoc test was used to compare the response between WT and <i>Ifnar1</i>^−/− mice given the same vaccination. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.