Proteomics approach used for phosphotyrosine profiling of <i>E. coli</i>.
- Anne-Marie Hansen (421550)
- Raghothama Chaerkady (156766)
- Jyoti Sharma (572854)
- J. Javier Díaz-Mejía (196503)
- Nidhi Tyagi (176982)
- Santosh Renuse (2131054)
- Harrys K. C. Jacob (421551)
- Sneha M. Pinto (421552)
- Nandini A. Sahasrabuddhe (421553)
- Min-Sik Kim (421554)
- Bernard Delanghe (421555)
- Narayanaswamy Srinivasan (85259)
- Andrew Emili (65325)
- James B. Kaper (248961)
- Akhilesh Pandey (14293)
Publication date
June 2013
DOIAbstract
<p>EHEC O157:H7 strain TUV93-0 and <i>E. coli</i> K12 strain MG1655 were grown to stationary phase in DMEM. Total protein was trypsin-digested, peptides purified using reversed-phased chromatography and subjected to immunoaffinity (IP)-based phosphotyrosine enrichment. Tyrosine phosphorylated peptides were identified by high resolution LC-MS/MS analysis using LTQ Orbitrap XL and Velos instruments.</p
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