Cellular map of prioritized gene hits as potential modulators of innate immunity.

<p>Cellular mapping of gene products of 96 gene hits out of 114 that modulated endogenous <i>IFNB1</i> expression either as Positive Regulators (71 PRs: Red) or as Negative Regulators (25 NRs: green) of the signaling pathway following SeV infection. PRs and NRs were positioned on a cell map and classified according to sub-cellular compartments and cellular function using annotation information from Gene Ontology, KEGG pathway, Uniprot, NCBI and Ingenuity pathway Analysis (IPA). The four functional groups are represented by different symbols: I - SeV specific (triangle), II - cytoplasmic dsRNA sensing (diamond), III - MAVS-dependent signaling (square) and IV - Nuclear import or transcription factor-dependent process (circle). Gene hits with knockdown efficiency confirmed by qRT-PCR with at least two independent shRNAs are highlighted in bold (41 PRs and 9NRs). The RLR and WNT signaling pathways are represented in light and dark blue respectively. Virus-induced activation of the WNT/CTNNB1 canonical-like pathway in feedback inhibition of antiviral innate responses is indicated in red.