PTPN12 modulates TrkB signaling in TrkB-SH-SY5Y cells.

<p>A) Western blot analyses for pY816-TrkB, TrkB, pERK1/2, pAKT and Vinculin levels in control and PTPN12 kd cells cultured for 24 h, starved for 4 h and stimulated for 5 min with 0, 1 or 10 ng/ml BDNF. Graphs show densitometric quantifications of pY816-TrkB compared to total TrkB, and pERK1/2 and pAKT compared to total Vinculin, for cells in absence of BDNF (basal level) or with 1 ng/ml BDNF (mean and S.E.M. of three, seven, and four independent experiments respectively). PTPN12 kd values were normalized to the level of the control for each BDNF concentration. NB: p-value = 0.076 for comparison of pAKT between control and PTPN12 kd both at 0 ng/ml BDNF. B) PTPN12 kd and control cells cultured as in A) in the absence of BDNF ± K252a were analyzed for pERK1/2 levels using GAPDH as a reference. Respective densitometric quantifications are shown as mean and S.E.M. of three independent experiments; PTPN12 kd is normalized to the level of the control in the absence or presence of K252a. Statistical analyses were performed using Student’s paired t-test comparing control and Ptpn12 kd, except where otherwise specified (*: p≤0.05; ***: p≤0.005). NB: p-value = 0.060 for comparison of pERK between control and PTPN12 kd both treated with K252.