E1A recruits hBre1 to HAdV early gene promoters, but HAdV chromatin is not monoubiquitinated by hBre1.

<p>A549 cells were infected with WT HAdV or ΔE1A HAdV for 20 hours. Chromatin immunoprecipitation (ChIP) was then performed with antibodies specific for the indicated proteins. DNA was probed via qRT-PCR for the presence of HAdV early gene promoters. Data was normalized to ΔE1A HAdV and a non-specific antibody control. Occupancy by RNA pol II and E1A (A), hBre1 complex members (B), and the indicated histone post-translational modifications (C) at HAdV early gene promoters are shown. In B, a statistically significant increase from mouse antibody ChIP is indicated (* P<0.01). n = 3.