Secreted IgM and membrane IgM modulation in response to oral DNA vaccination.

<p>Trout were orally vaccinated and sampled as described in the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066118#pone-0066118-g004" target="_blank">Figure 4</a> and levels of secreted IgM (A) and membrane IgM (B) transcription in the different segments were studied through real time PCR. Data are shown as the mean relative gene expression normalized to the transcription of the house-keeping gene EF-1α ± SD (n = 6). The relative significance of differences between fish vaccinated with either the empty plasmid or empty microspheres and vaccinated fish at each segment of the digestive tract was determined through a one-way ANOVA and is shown above the bars as *.