A: FRAP microscopy of fluorescently labeled Fab fragments of IgG.

<p>For a typical FRAP experiment performed on a fibrin network, the regions of interest (ROIs, radius of 27 m) before, immediately after photobleaching, and after 98 s are shown (green circle). Normalized fluorescence recovery for the corresponding ROIs and their fits according to a model (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003095#pcbi.1003095.e163" target="_blank">Equation (7)</a>, ) are shown by symbols and a line, respectively. Fluorescence intensity values before () and after () photobleaching, and at the end of the experiment (I_∞), are shown. For each sample, FRAP measurements were repeated 3 times in 8 different locations over the sample and the arithmetic mean of the intensity curves was taken. B: Hydrodynamic diameter measurements by dynamic light scattering: circles denote -thrombin and squares denote Fab IgG.