IDO ablation enhances influenza-specific CD8 T cell responses to PR8 infection.

<p>B6 (WT) or IDO1-KO mice with B6 backgrounds were infected with PR8 virus (50 PFU, i/n) at sub-lethal doses, and 10 days after infection bronchial alveolar lavage (BAL) cells and T cells were counted. <b>A.</b> Representative FACS gating and analysis strategy to detect NP₃₆₆-specific CD8 T cells using tetramers (upper panel) and intracellular cytokine (IFNγ, IL-10) expression after peptide specific stimulation for 2 hrs <i>ex vivo</i> (lower panel). <b>B–F.</b> Numbers of total BAL (B) and NP₃₆₆ (C), PA (D), PB1 (E) CD8 and NP₃₁₁ (F) CD4 specific T cells. Data were analyzed using Student's unpaired <i>t</i> test; *p<0.05, **p<0.01, ***p<0.001, ns, not significant.