Identification of the circulation of <i>gata1⁺</i> blood cells through V2^LP and V4^LP inter-organ vessels.

<p>Time-lapse two-photon microscopy images of <i>Tg(gata1:DsRed);Tg(fli1:egfp)</i> larvae at 5 dpf (V1^LP, V2^LP, V4^LP) and at 6 dpf (V3^LP). No circulation of <i>gata1⁺</i> blood cells was observed within V1^LP or V3^LP vessels during the 15 min. or 14 min. imaging periods, respectively. In contrast, the circulation of <i>gata1⁺</i> blood cells was clearly observed both within V2^LP and V4^LP vessels during the 7 min. imaging period. The 3D rendered (3D) and/or Z-slices are shown. EGFP (green): <i>fli1⁺</i> vessels; DsRed (magenta): <i>gata1⁺</i> blood cells. The time-stamp (min: minute, sec: second) is indicated at the bottom right in each bottom panel. Scale bars: 30 µm.