In planta PTD-GFP transplastomic tobacco leaves after <i>E. carotovora</i> infection, imaged by confocal microscope.

<p>Bacterial suspension (1.0×10⁵ cells) of <i>E. carotovora</i> was injected into the tobacco leaves with a syringe. The infected area (1 cm² disk) of individual plant was punched-out from the leaves (five leaf discs for each point) and analyzed by confocal microscopy after 24 or 48 hr of <i>E. carotovora</i> infection. Representative images are from 57 stored images out of 175 observations of PTD-GFP chloroplasts after 24 (A) or 48 hr (B) of E. carotovora infection. (C) Representative images are from 50 stored images among 167 observations of PTD-GFP leaf cells without <i>E. carotovora</i> infection. Bars represent 10 µm.