Expression of cell cycle regulatory proteins following RAD001 and IR treatment.

<p>Bladder cancer cells were treated with RAD001, ionizing radiation (IR) or the combined treatment. They were lysed 24 hours after treatment as described in Methods. Western blot analysis for Cyclin D1, p27kip1 and p21 in KU7 cells and normalized in lower panels as a function of the actin level measured in parallel. Similar results were obtained for all cell lines tested, UM-UC3, UM-UC6 and 253J-BV (not shown).