Rescue of the <i>DBHD^−/−</i> growth defects by nutrients.

<p>(A) <i>DBHD^−/−</i> larvae were sensitive to yeast supply. NF: normal food; Star: starvation, normal food without yeast. All samples were picked at Day 8 after hatching from eggs. (B) The developmental profiles of flies cultured on two kinds of nutritious foods. yeast paste: the baker’s yeast powder was mixed with water and supplied on agar plate. leucine: normal food supplemented with 100 mM leucine. Fifty newly hatched larvae for each genotype were picked. Numbers of the heterozygotes (left) and <i>DBHD^−/−</i> were separated by slashes. (C) Examples of four dead <i>DBHD^−/−</i> pharates cultured on yeast pastes. (D) Rescue effects of leucine. Animals aged for 7 days after hatching were imaged. NF: normal food; leu: normal food with 100 mM leucine; rapa: normal food with 1 µM rapamycin; leu+rapa: normal food with 100 mM leucine and 1 µM rapamycin. (D) Free amino acids analysis of the larvae. The amino acids levels are displayed as milligram per gram of body weight (mg/g). The amounts of larvae for each experiment are listed in the parenthesis.