SNAI1 overexpression in MCF10A cells induced a dramatic change in gene expression profile.

<p>(A) The microarray discovered 966 genes differentially expressed in MCF10A and MCF10A-SNAI1 cells (fold change >2, <i>p</i>-value <0.01). 966 genes were subjected to GO analysis using Gene Spring GX program to gain insights on biological functions of the genes. Majority of differentially regulated genes were shown to be involved in signaling. FDR-corrected p-values were displayed and all significantly enriched processes were highlighted by bold characters. (B) Using the functional annotation clustering tool available in DAVID bioinformatics database, genes were classified into functionally related gene groups. Selected sets of genes under the categories like signaling and response were displayed. (C) In the heat map, genes involved in GO term, regulation of cell death were shown. Many of them were revealed to be associated with signaling pathways like NF-κB (red arrow), Wnt (yellow arrow), TGFß (blue arrow), and Notch/STAT (green arrow).