Flow cytometric analysis of Tregs in spleen, lymph nodes and thymus of ovariectomized mice.
- Ana Paula Ligeiro de Oliveira (422582)
- Jean Pierre Schatzmann Peron (422583)
- Adriana Lino Santos Franco (422584)
- Beatriz Acceturi Golega (422585)
- Rodolfo Paula Vieira (422586)
- Olga Célia Martinez Ibanez (422587)
- Orlando Garcia Ribeiro (422588)
- Wafa Hanna Koury Cabrera (422589)
- Marcelo De Franco (422590)
- Ricardo Martins Oliveira-Filho (52900)
- Luiz Vicente Rizzo (343545)
- Bernardo Boris Vargaftig (422591)
- Wothan Tavares de Lima (52899)
Publication date
June 2013
DOIAbstract
<p>7 days after ovariectomy, OVx and Sham mice were sacrificed and spleen, lymph nodes and thymus cells were submitted to flow cytometry protocol and stained for CD4– FITC, CD8–PeCy5 and Foxp3– PE. In A) Zebra plots demonstrate the gates used and the percentage of positive cells. In B relative and C absolute numbers of Tregs. Graphepresentative of three independent experiments. One way-ANOVA p<0.01. n = 5 animals per group.</p
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