Western blot for PDK1/Akt pathway and gene expression of E2fs.

<p>(A) Protein levels of the PDK1/Akt pathway in wild-type (WT) and PPARβ-null (KO) mice. Hepatic gene expression levels of (B) E2f1, (C) E2f2, (D) E2f7, and (E) E2f8 over a time course from 0 to 72 hours after PH by qPCR (n = 3). (F) Differential analysis results for E2fs. Expression plot shows clear differences in the expression of E2f1, 2, 7, 8 between KO versus WT mice, measured in fragments per kilobase of exon per million fragments mapped (FPKM). Expression of a transcript is proportional to the number of reads sequenced from that transcript after normalizing for that transcript’s length. Means ± SD are graphed. * <i>p</i><0.05.