Lineage tracing of adult <i>Shh⁺</i> cells.

<p>(A–D) <i>Shh^ERCre/+</i>;<i>R26^Tom/+</i> mice were pulsed with tamoxifen at P50–54 and sacrificed at P80 (A–C) and P136 (D). TdTomato expression in the tongue (A, C, D), and the magnified brightfield (B) and fluorescent (C) images of boxed area (A) are shown, demonstrating that labeled <i>Shh</i>-derivatives disappeared over the next 3 months. (E–I) TdTomato/RFP stained sections from <i>Shh^ERCre/+</i>;<i>R26^Tom/+</i> mice FG (E) and CV (F–I) papillae shown in Fig. 4A–C, with indicated markers. (G–I) Taste bud is outlined by dotted white line. Scale bars = 20 µm (E, G–I), 50 µm (F), and 500 µm (A–D).