Infection assay in Rev-CEM cells for various single-cycle HIV-1 virions.

<p>(A) Negative control in the absence of infection, which was used to gate cell populations in infected cells. (B) A representative cytogram for Rev-CEM cells infected by single-cycle virions generated with 2 μg envelope. The virions were harvested 18 hours post transfection with media change six hours post transfection. (C) and (D) show C_i.p. and infectivity in Rev-CEM cells for virions produced with various inputs of envelope plasmid pcDNA3.1REC. Circles and triangles are for virions collected 18 and 48 hours post transfection, respectively, with filled symbols representing samples with media change and open symbols representing samples without media change. Error bars are standard deviations from three independent replicates of the same experiments.