Examples of intergenic HPV16 DNA integration sites.

<p>(A) Tumor T3966 has one transcriptionally active HPV16 integration site 3966_DJ1 (TA-group 1), which is located between the cellular genes <i>RASSF6</i> and <i>IL8</i>, and has the same orientation as the downstream gene <i>IL8</i>. In the fusion transcript 3966_RJ, the viral exon is spliced to an intergenic alternative cellular exon. (B) Tumor T3256 (TA-group 2) has three HPV16 integration sites, one (3256_DJ1) on chromosome 3 (not shown) and two (DJ2 and DJ3) on chromosome 10q24.2 between the cellular genes <i>LOXL4</i> and <i>PYROXD2.</i> Only 3256_DJ3 is transcribed. The fusion transcript 3256_RJ is in opposite orientation to the downstream gene <i>PYROXD2</i>. (C) In the chromosome region 13q22.1-2, five HPV16 integration sites identified in four independent DNA samples are located in the large intergenic region between the cellular genes <i>KLF5</i> and <i>KLF12</i>. Only one integrated HPV16 DNA (4046_DJ1) is transcribed.