Shortened kinetics of antigen presentation <i>in vivo</i> in lentivector-boosted mice.

<p>B6 mice were primed, or primed and boosted with Lv-OVA, and then were adoptively transferred i.v. with 2×10⁶ CFSE-labeled OT-I (<b>A</b>) or OT-II (<b>B</b>) splenocytes at the indicated days. Three days later the LNs were harvested and the CFSE dilution was assessed by flow cytometry to analyze the proliferation of the transferred CD8⁺ or CD4⁺ T cells, respectively. Results are shown as representative flow plots gating on dividing CD8⁺ or CD4⁺ lymphocytes; numbers indicate the percentages of dividing cells and represent the mean of three mice per group for each time point ± SE. One representative out of 2 independent experiments is depicted. *, <i>P</i><0.01, primed mice versus boosted mice at the time points indicated.