TEM4 regulates persistence of endothelial cell migration.

<p>(A) Wind-Rose plots depicting migratory tracks of six individual migrating cells in each experimental group. Values on x and y scales are arbitrary. (B) Persistence of two-dimensional cellular migration of HUVECs expressing NS, TEM4, or RhoC shRNAs. Data shown are the mean ± s.e.m. measured from 18–27 individual cells in 3–5 independent experiments. Cells expressing plasmid-based shRNA constructs were identified by monitoring GFP or mCherry fluorescence (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066260#pone.0066260.s001" target="_blank">Fig. S1</a>). (C) Western blot confirming knockdown of TEM4 and RhoC expression levels by lentivirus-based RNAi constructs. NS; non-specific shRNA.