TEM4 and RhoC are required to maintain proper actin cytoskeleton architecture in lamella.

<p>(A) Actin filaments were visualized by staining with phalloidin and α-actinin 1 in NS control or cells depleted of TEM4 and RhoC. Cells were prepermeabilized with Triton X-100 to remove cytosolic α-actinin 1. The whole cell and an isolated area of the leading edge are shown. Scale bar, 10 µm. (B) Depletion of TEM4 or RhoC increases the number of cells with tight actin/α-actinin bundles. Data are mean ± s.e.m. obtained from 42–57 cells in three independent experiments.