Effects of fibronectin and collagen on the immunophenotype of ECFCs.

<p>ECFCs expanded on fibronectin or collagen showed a similar immunophenotype. On the left are reported flow cytometric histograms showing that, independently from the substrate used for cell culture, ECFCs did not express CD45 and CD14, while they expressed at similar levels CD31, VEGFR2, CD144, CD146, CD54 and CXCR4. On the right are reported immunofluorescence photographs showing that ECFCs expanded on both substrates incorporated Dil-ac-LDL and bound lectin UEA-1. Photographs were obtained using an an Olympus Fluoview FV1000 confocal microscope, x40 magnification. One of three representative experiments is reported.