<p>Gene expression was measured by qPCR in WT or p65 null MEFs treated or not with TNF-α (mean ± SEM of 4–5 independent experiments). <i>n.d.: not detected, values sharing a different letter are statistically different at p<0.05 (one-way ANOVA followed by Tukey-Kramer post hoc test).</i></p
RAW264.7 cells were treated with the f NF-κB activation inhibitor and MAPKs activation inhibitors. A...
<p>Cells were incubated in the presence of TNF-α (40 ng/ml) for 4 h. Gene expression was measured by...
<p>Data are normalized to the vehicle treated vector group. *—p<0.01 for the effect of TNFα in the v...
<p>Measures of gene expression were performed by quantitative RT-PCR for transcription factors <i>nf...
<p>(A) Western blot of NF-κB family members in cytoplasmic and nuclear protein extracts (15 µg/sampl...
<p><b>A & B</b>, U251-TR/<i>sh-p65</i> cells were incubated with tetracycline (Tet) for 48 h prior t...
<p>Normalized data show a significant increase in four genes in TNF-α-stimulated cells. On the other...
Treatment groups and sampling for RNA extraction as described in Fig 2. Expression value of each gen...
<p>(A) TNF-induced chemokines in SKOV-3 cells. After isolating total RNA, PCR array was performed us...
<p>(<b>A,C</b>) Protein content of transcription factor precursor p105 and its active form, p50 (A) ...
<p>(A) 293 cells were treated with the P-TEFb inhibitor DRB for 6 h and endogenous <i>MYB</i> expres...
<p>(A) HeLa cells (5×10<sup>6</sup>) were transfected with siRNA control or siRNA p65 (200 pmol). Fo...
<p>(A) A549 cells were transfected with either pORF3-EGFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimula...
<p>qPCR was used to confirm the upregulation or induction of chemokine expression by IFNγ and/or TNF...
<p>Nuclear NF-κB-p65 levels were measured by ELISA in cells stimulated with CSE (<b><i>C</i></b>), n...
RAW264.7 cells were treated with the f NF-κB activation inhibitor and MAPKs activation inhibitors. A...
<p>Cells were incubated in the presence of TNF-α (40 ng/ml) for 4 h. Gene expression was measured by...
<p>Data are normalized to the vehicle treated vector group. *—p<0.01 for the effect of TNFα in the v...
<p>Measures of gene expression were performed by quantitative RT-PCR for transcription factors <i>nf...
<p>(A) Western blot of NF-κB family members in cytoplasmic and nuclear protein extracts (15 µg/sampl...
<p><b>A & B</b>, U251-TR/<i>sh-p65</i> cells were incubated with tetracycline (Tet) for 48 h prior t...
<p>Normalized data show a significant increase in four genes in TNF-α-stimulated cells. On the other...
Treatment groups and sampling for RNA extraction as described in Fig 2. Expression value of each gen...
<p>(A) TNF-induced chemokines in SKOV-3 cells. After isolating total RNA, PCR array was performed us...
<p>(<b>A,C</b>) Protein content of transcription factor precursor p105 and its active form, p50 (A) ...
<p>(A) 293 cells were treated with the P-TEFb inhibitor DRB for 6 h and endogenous <i>MYB</i> expres...
<p>(A) HeLa cells (5×10<sup>6</sup>) were transfected with siRNA control or siRNA p65 (200 pmol). Fo...
<p>(A) A549 cells were transfected with either pORF3-EGFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimula...
<p>qPCR was used to confirm the upregulation or induction of chemokine expression by IFNγ and/or TNF...
<p>Nuclear NF-κB-p65 levels were measured by ELISA in cells stimulated with CSE (<b><i>C</i></b>), n...
RAW264.7 cells were treated with the f NF-κB activation inhibitor and MAPKs activation inhibitors. A...
<p>Cells were incubated in the presence of TNF-α (40 ng/ml) for 4 h. Gene expression was measured by...
<p>Data are normalized to the vehicle treated vector group. *—p<0.01 for the effect of TNFα in the v...