Piperine induced caspase activation in prostate cancer cell lines.

<p>A NIR-FLIVO 747-conjugated poly-caspase probe, which is a cell-permeable fluorescent detector of active caspases, was employed to determine whether piperine activates caspase in prostate cancer cells. The LNCaP and PC-3 cell lines were treated with piperine and then tested for caspase activation. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065889#s3" target="_blank">Results</a> showed that piperine induced global caspase activation in both LNCaP (A) and PC-3 cells (B) as early as 24 hours. Experiments were repeated four times and obtained similar results as shown in this representative figure.