SCA+CT yields differences between <i>ex vivo</i> tissue types.

<p>Representative H&E images (column 1) of tumor, tumor+muscle (T+M), muscle, and adipose tissue and their corresponding fluorescence microendoscopy (FM) image (column 2) are shown (<b>a</b>)–(<b>d</b>) respectively. Nuclei were isolated and quantified by using SCA+CT and were overlaid onto the original image, which is illustrated in column 4. Nuclei that are greater than 8 µm in diameter are false colored red, while those that are equal to or less than 8 µm are false colored green. In column 3, the contrast of the original panel was adjusted to match the SCA+CT overlay in column 4. Scale bar 200 µm. The nuclear size (<b>e</b>), total density (<b>f</b>), density of the smaller nuclei (green, (<b>g</b>)), and density of the larger nuclei (red, (<b>h</b>)) were calculated for a cohort of 8 tumor, 6 T+M, and 13 muscle images. For diameter, the parenthetical values indicate the number of nuclei whose diameter was quantified. For density, the parenthetical values indicate the number of images in which the density was calculated. In (<b>e</b>) the vertical dotted red line corresponds to an 8 µm diameter, and the horizontal color bars show the mean and standard deviation for each variable. Significant differences are seen between tumor and muscle images for total density and the density of the smaller nuclei (green). (<b>i</b>) An ROC curve was generated using total density, density of the smaller nuclei (green), and density of the larger nuclei (red) as classifiers. The area under the curve (AUC) for each of the classifiers is 0.84, 0.84, and 0.73 respectively.